FastQCFastQC Report
Wed 2 Dec 2015
MG.R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14699402
Sequences flagged as poor quality0
Sequence length103
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCTACTTCGGA2183351.4853325325751348Illumina Paired End PCR Primer 2 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG318450.078.043723
ATCGGAA334600.074.449882
GATCGGA335750.074.267881
AGAGCGG335250.073.946178
AAGAGCG341950.072.5824057
GAGCGGT345000.071.701739
GGAAGAG346850.071.696875
CGGAAGA354050.070.2322164
GAAGAGC372700.066.6596
CTCTACT280600.041.3736138-39
TCGTATG217200.041.10566352-53
TCTCTAC280400.041.0658838-39
ATCTCTA286050.040.65439636-37
GATCTCT301500.038.5791536-37
AGGAATG322000.038.51649520-21
CGTATGC223250.038.0471354-55
TCTACTT305700.037.7624140-41
TATCGTA159800.037.6780150-51
GGAATGC333000.037.0189622-23
CTTCGGA309100.036.85553744-45
Produced by FastQC (version 0.11.3)