FastQCFastQC Report
Wed 2 Dec 2015
MG.R1.preprocessed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.R1.preprocessed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13108258
Sequences flagged as poor quality0
Sequence length40-103
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCAA149350.011.8599178
AACCCCA149900.011.7887927
AAACCCC150900.011.6832456
CCCCAAA157850.011.2737131
AAAACCC159350.010.9857925
CCCAAAA166300.010.6262182
CCAAAAC181050.09.8519413
CAAAACC205800.08.7073424
AACCCCT49350.06.792676430-31
Produced by FastQC (version 0.11.3)