Experiment: Current- and Voltage-clamp (cell characterization)
Cell Type: Organoids from Luxemburg (5 days after dissociation, Neuro-Cult Kit dissociated at day 60)
Date: 17-10-17
Temp: 33-35°C
External solution: Dopamine Neurons
Internal solution: Dopamine
Drugs:----
Researcher: Marcelo

Cell #1
Cm= 9.3 pF 
Rm= 308 MOhms
Ra= 14 MOhms
Current-Steps
2017_10_17_0000.abf started at 01:24:11 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0001.abf restarted at 01:25:42 stopwatch time.
Vh= -60mV
Lost

Cell #2
Cm= 24 pF 
Rm= 468 MOhms
Ra= 14 MOhms
Current-Steps
2017_10_17_0003.abf started at 01:52:21 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0004.abf restarted at 01:53:39 stopwatch time.
Vh= -60mV
Lost

Cell #3
Cm= 23 pF 
Rm= 1200 MOhms
Ra= 17 MOhms
Current-Steps
2017_10_17_0005.abf started at 02:12:52 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0006.abf started at 02:13:52 stopwatch time.
2017_10_17_0007.abf started at 02:14:18 stopwatch time.
Vh= -60mV
Stop

Cell #4
Cm= 26 pF 
Rm= 2000 MOhms
Ra= 19 MOhms
Current-Steps
2017_10_17_0009.abf started at 02:54:58 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0010.abf started at 02:55:48 stopwatch time.
Vh= -60mV
Stop

Cell #5
Cm= 10 pF 
Rm= 1600 MOhms
Ra= 13 MOhms
Current-Steps
2017_10_17_0011.abf started at 03:22:50 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0012.abf started at 03:23:40 stopwatch time.
Vh= -60mV
Lost

Cell #6
Cm= 10 pF 
Rm= 943 MOhms
Ra= 21 MOhms
Current-Steps
2017_10_17_0013.abf started at 03:35:38 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0014.abf started at 03:36:38 stopwatch time.
Vh= -60mV
Lost

Cell #7
Cm= 12 pF 
Rm= 372 MOhms
Ra= 18 MOhms
Current-Steps
2017_10_17_0015.abf started at 03:50:20 stopwatch time.
Vh= -60mV
Lost

Cell #8
Cm= 17 pF 
Rm= 635 MOhms
Ra= 20 MOhms
Current-Steps
2017_10_17_0016.abf started at 05:49:08 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_17_0017.abf started at 05:50:28 stopwatch time.
Vh= -60mV
Lost

Cell #9
Cm= 10 pF 
Rm= 350 MOhms
Ra= 14 MOhms
Current-Steps
2017_10_17_0018.abf restarted at 06:08:26 stopwatch time.
Ionic Currents (Vh=-60mV) (INa.pro)
2017_10_17_0019.abf started at 06:09:33 stopwatch time.
Vh= -60mV
Lost

Cell #10
Cm= 36 pF 
Rm= 810 MOhms
Ra= 10 MOhms
Current-Steps
2017_10_17_0022.abf started at 07:09:07 stopwatch time.
Ionic Currents (Vh=-60mV) (INa.pro)
2017_10_17_0023.abf started at 07:09:51 stopwatch time.
Vh= -60mV
Stop

Cell #11
Cm= 11 pF 
Rm= 544 MOhms
Ra= 21 MOhms
Current-Steps
2017_10_17_0024.abf started at 07:34:54 stopwatch time.
Ionic Currents (Vh=-60mV) (INa.pro)
2017_10_17_0025.abf started at 07:35:53 stopwatch time.
Vh= -60mV
Stop

Cell #12
Cm= 16 pF 
Rm= 812 MOhms
Ra= 8 MOhms
Spontaneous activity
2017_10_17_0027.abf started at 07:47:55 stopwatch time.
Vh= -60mV
Lost

Cell #13
Cm= 15 pF 
Rm= 2300 MOhms
Ra= 20 MOhms
Current-Steps
2017_10_17_0028.abf restarted at 08:02:08 stopwatch time.
Spontaneous activity
2017_10_17_0029.abf started at 08:03:26 stopwatch time.
2017_10_17_0030.abf started at 08:05:48 stopwatch time.
Ionic Currents (Vh=-60mV) (INa.pro)
2017_10_17_0031.abf started at 08:14:22 stopwatch time.
Vh= -60mV
Stop