Experiment: Current- and Voltage-clamp (cell characterization)
Cell Type: Organoids from Luxemburg (7 days after dissociation, cutting + digestion)
Date: 21-09-17
Temp: 33-35°C
External solution: Dopamine Neurons
Internal solution: Dopamine
Drugs:----
Researcher: Marcelo

26 Cells have been patched. 2 cells recorded. 1 cell with activity (Cell#1)
Cell #1
Cm= 24 pF 
Rm= 805 MOhms
Ra= 14 MOhms
GapFree Current-Clamp
2017_09_21_0000.abf started at 00:41:31 stopwatch time.
2017_09_21_0001.abf started at 00:42:31 stopwatch time.
Ra= 13 MOhms
GapFree Voltage-Clamp (No synaptic currents)
2017_09_21_0002.abf started at 00:43:19 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_09_21_0003.abf started at 00:45:35 stopwatch time.
INa-current (INa.pro)
2017_09_21_0004.abf started at 00:46:19 stopwatch time.
Ra= 16 MOhms
GapFree Current-Clamp
2017_09_21_0005.abf started at 00:47:11 stopwatch time.
2017_09_21_0006.abf started at 00:48:07 stopwatch time. (injected current)
2017_09_21_0007.abf started at 00:48:55 stopwatch time.
Ra= 13 MOhms
Vh= -60mV
Lost

Cell #2
Cm= 407 pF 
Rm= 78 MOhms
Ra= 12 MOhms
GapFree Current-Clamp (No spontaneous activity)
2017_09_21_0008.abf started at 01:13:44 stopwatch time. 
Ra= 10 MOhms
GapFree Voltage-Clamp (No synaptic currents)
2017_09_21_0009.abf started at 01:14:40 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_09_21_0010.abf started at 01:15:57 stopwatch time.
Ra= 10 MOhms
Current Steps
2017_09_21_0011.abf started at 01:16:46 stopwatch time.
Ra= 9 MOhms
Vh= -60mV
Stop