Experiment: Current- and Voltage-clamp (cell characterization)
Cell Type: Organoids (Mutant) from Luxemburg (15 days after dissociation, Neuro-Cult Kit dissociated at day 60)
Date: 24-10-17
Temp: 33-35°C
External solution: Dopamine Neurons
Internal solution: Dopamine
Drugs:----
Researcher: Marcelo

Cell #1
Cm= 16 pF 
Rm= 1100 MOhms
Ra= 23 MOhms
GapFree Current Calmp
2017_10_24_0000.abf started at 00:39:12 stopwatch time.
2017_10_24_0001.abf started at 00:40:52 stopwatch time.
Vh= -60mV
Lost

Cell #2
Cm= 19 pF 
Rm= 520 MOhms
Ra= 8 MOhms
Current Step
2017_10_24_0003.abf started at 00:54:01 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_24_0004.abf started at 00:57:59 stopwatch time.
Ra= 8 MOhms
Vh= -60mV
Stop

Cell #3
Cm= 12 pF 
Rm= 735 MOhms
Ra= 10 MOhms
Current Step
2017_10_24_0005.abf restarted at 01:08:19 stopwatch time.
Vh= -60mV
Lost

Cell #4
Cm= 12 pF 
Rm= 735 MOhms
Ra= 10 MOhms
Spontaneous APs 
2017_10_24_0006.abf started at 01:19:02 stopwatch time.
2017_10_24_0007.abf started at 01:21:39 stopwatch time.
Current Step
2017_10_24_0008.abf started at 01:22:45 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_24_0009.abf started at 01:23:32 stopwatch time.
Ra= 13 MOhms
Vh= -60mV
Stop

Cell #5
Cm= 33 pF 
Rm= 430 MOhms
Ra= 13 MOhms
Spontaneous APs 
2017_10_24_0010.abf started at 01:39:18 stopwatch time.
Spontaneous ACs (Voltage-clamp)
2017_10_24_0011.abf started at 01:41:23 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_24_0012.abf started at 01:42:59 stopwatch time.
Current Step
2017_10_24_0013.abf started at 01:43:51 stopwatch time.
2017-10-24 11:11:36 Last Trial was saved to file 2017_10_24_0014.abf.
Ra= 16 MOhms
Vh= -60mV
Stop

Cell #6
Cm= 15 pF 
Rm= 468 MOhms
Ra= 28 MOhms
Spontaneous APs 
2017_10_24_0015.abf started at 02:14:52 stopwatch time.
Ra= 23 MOhms
Vh= -60mV
Lost

Cell #7
Cm= 22 pF 
Rm= 675 MOhms
Ra= 8 MOhms
Spontaneous APs (in BURST!!)
2017_10_24_0016.abf started at 02:23:04 stopwatch time.
Ionic Currents (Vh=-60mV) (Ica-HP80.pro)
2017_10_24_0017.abf started at 02:23:04 stopwatch time.
Ra= 7 MOhms
Vh= -60mV
Lost

Cell #8
Cm= 31 pF 
Rm= 619 MOhms
Ra= 11 MOhms
Spontaneous APs
2017_10_24_0018.abf started at 00:24:47 stopwatch time.
Spontanous ACs
2017_10_24_0019.abf started at 00:26:23 stopwatch time.
Ra= 10 MOhms
Vh= -60mV
Stop

Cell #9
Cm= 20 pF 
Rm= 754 MOhms
Ra= 26 MOhms
Spontaneous APs
2017_10_24_0020.abf started at 00:44:11 stopwatch time.
Spontanous ACs
2017_10_24_0021.abf started at 00:47:05 stopwatch time.
Vh= -60mV
Lost

Cell #10
Cm= 19 pF 
Rm= 848 MOhms
Ra= 14 MOhms
Spontaneous APs
2017_10_24_0022.abf started at 00:54:52 stopwatch time.
Vh= -60mV
Stop