Species:	Human (Homo sapiens GRCh38/hg38)										
Input:	ACCTGCAGCAGGGCCAGGGCCCAGGTGAAACGGATGCGACAGCAGCGGAGAGAAGAGCGGGAGGCAAAGACGCCAGCCTGGT										
PAM:	NGG										
Target site length:	20										
Target site 5' limitation:	NN										
Target site 3' limitation:	NN										
Core length:	12										
Core MM:	2										
Total MM:	4										
											
											
T3	TGAAACGGATGCGACAGCAGCGG	956	CRISPRater score	0.73011224							
Oligo adding fwd	CACCgTGAAACGGATGCGACAGCAG										
Oligo adding rev	AAACCTGCTGTCGCATCCGTTTCAC										
Oligo substituting fwd	CACCgGAAACGGATGCGACAGCAG										
Oligo substituting rev	AAACCTGCTGTCGCATCCGTTTCC										
Chromosome	start	end	strand	MM	target_seq	PAM	alignment	distance	position	gene name	gene id
chr16	28482128	28482150	+	0	TGAAACGGATGCGACAGCAG	CGG	||||||||[||||||||||||]PAM	0	Exonic	CLN3	ENSG00000188603
chr17	45768365	45768387	-	4	TATTAGGGATGCGACAGCAG	CGG	|---|-||[||||||||||||]PAM	15893	Intronic	CRHR1	ENSG00000120088
chr1	161889371	161889393	-	3	TGGAAGGGAAGCGACAGCAG	AGG	||-||-||[|-||||||||||]PAM	1440	Intronic	RP11-456P18.2	ENSG00000229808
chrX	31281990	31282012	+	4	TGAGAGTCATGCGACAGCAG	TGG	|||-|---[||||||||||||]PAM	15038	Intronic	DMD	ENSG00000198947
chr12	111427500	111427522	+	4	TGACGGGGAGGCGACAGCAG	TGG	|||---||[|-||||||||||]PAM	7340	Intronic	SH2B3	ENSG00000111252
chr2	199988604	199988626	-	3	TGATAGGGATGAGACAGCAG	TGG	|||-|-||[|||-||||||||]PAM	17117	Intronic	C2orf47	ENSG00000162972
chr11	123589985	123590007	-	4	GGACAGGGATGAGACAGCAG	TGG	-||-|-||[|||-||||||||]PAM	4075	Intronic	GRAMD1B	ENSG00000023171
chr2	222769658	222769680	-	4	TCATAAGGATGAGACAGCAG	AGG	|-|-|-||[|||-||||||||]PAM	23413	Intergenic	AC016712.3	ENSG00000264133
chr5	123512277	123512299	+	4	TGCATCGGTGGCGACAGCAG	AGG	||-|-|||[--||||||||||]PAM	0	Exonic	CSNK1G3	ENSG00000151292
chr19	33082109	33082131	+	4	TGGCATGGATGGGACAGCAG	TGG	||--|-||[|||-||||||||]PAM	843	Intronic	GPATCH1	ENSG00000076650
chrX	14212247	14212269	+	4	TGCACTGGATGGGACAGCAG	TGG	||-|--||[|||-||||||||]PAM	32113	Intergenic	GS1-257G1.1	ENSG00000233247
chr6	14781157	14781179	+	4	AGAAGCAGATGAGACAGCAG	AGG	-|||-|-|[|||-||||||||]PAM	8811	Intronic	RP11-146I2.1	ENSG00000234261
chr6	159714180	159714202	-	4	TGTGACAGATGGGACAGCAG	AGG	||--||-|[|||-||||||||]PAM	195	Intronic	HNRNPH1P1	ENSG00000220305
chr21	5070571	5070593	+	4	TGCCACTGATGGGACAGCAG	TGG	||--||-|[|||-||||||||]PAM	2865	Intergenic	CH507-9B2.8	ENSG00000279687
chr21	44191553	44191575	-	4	TGCCACTGATGGGACAGCAG	TGG	||--||-|[|||-||||||||]PAM	9715	Intergenic	AP001057.1	ENSG00000232124
chr10	79638949	79638971	+	4	TGTAGCTGATGTGACAGCAG	TGG	||-|-|-|[|||-||||||||]PAM	6761	Intergenic	RP11-589B3.6	ENSG00000226676
chr1	216813928	216813950	-	3	TGAATCAGATGCCACAGCAG	AGG	||||-|-|[||||-|||||||]PAM	8561	Intronic	ESRRG	ENSG00000196482
chr14	29559093	29559115	+	3	TGAAACAGCTGTGACAGCAG	TGG	||||||-|[-||-||||||||]PAM	17364	Intergenic	PRKD1	ENSG00000184304
chr1	9788988	9789010	-	4	TCCAACGCATGCCACAGCAG	GGG	|--||||-[||||-|||||||]PAM	7748	Intronic	MZT1P1	ENSG00000271264
chr21	38468546	38468568	+	4	TAAAACCCATGTGACAGCAG	AGG	|-||||--[|||-||||||||]PAM	2207	Intronic	ERG	ENSG00000157554
chr16	82792043	82792065	-	4	TCAAAAGGAGGGGACAGCAG	CGG	|-|||-||[|-|-||||||||]PAM	18554	Intronic	RP11-22H5.2	ENSG00000260862
chr6	114701947	114701969	-	4	TGAAAGCTATGGGACAGCAG	AGG	|||||---[|||-||||||||]PAM	76540	Intergenic	AL590964.1	ENSG00000273817
chr6	143147724	143147746	-	4	TAAAACAGAGGTGACAGCAG	AGG	|-||||-|[|-|-||||||||]PAM	10734	Intronic	AIG1	ENSG00000146416
chr1	110713196	110713218	-	4	TCAAACAGAAGGGACAGCAG	GGG	|-||||-|[|-|-||||||||]PAM	38163	Intergenic	KCNA3	ENSG00000177272
chr22	45760904	45760926	+	4	TTAAAAGGTTGCTACAGCAG	TGG	|-|||-||[-|||-|||||||]PAM	306	Intronic	MIR4762	ENSG00000264160
chr4	132882422	132882444	-	4	TGCAAGGGTTGCAACAGCAG	TGG	||-||-||[-|||-|||||||]PAM	11905	Intronic	RP11-404I7.2	ENSG00000251488
chr1	27318945	27318967	+	4	TGATAAGGAAGCAACAGCAG	AGG	|||-|-||[|-||-|||||||]PAM	3178	Intergenic	TMEM222	ENSG00000186501
chr8	80931368	80931390	-	4	GGAAAAGGATACAACAGCAG	AGG	-||||-||[||-|-|||||||]PAM	36420	Intergenic	PAG1	ENSG00000076641
chrX	105390110	105390132	-	4	TTAAAGGGATACAACAGCAG	AGG	|-|||-||[||-|-|||||||]PAM	15515	Intronic	KCTD9P2	ENSG00000181819
chr10	93609539	93609561	+	3	TGAAACTGATGTGGCAGCAG	AGG	||||||-|[|||-|-||||||]PAM	840	Intergenic	AL356214.1	ENSG00000221352
chr21	39643506	39643528	-	4	TAAAACAGCTGCGAAAGCAG	AGG	|-||||-|[-|||||-|||||]PAM	2864	Intronic	B3GALT5	ENSG00000183778
chr20	6077006	6077028	+	4	GGAAAGGGATGTGCCAGCAG	TGG	-||||-||[|||-|-||||||]PAM	0	Exonic	FERMT1	ENSG00000101311
chr9	113355192	113355214	-	4	TGAAAGTGATGACACAGCAG	TGG	|||||--|[|||--|||||||]PAM	854	Intronic	BSPRY	ENSG00000119411
chr15	74808312	74808334	+	4	TGAAGCTGAAGCGAAAGCAG	GGG	||||-|-|[|-||||-|||||]PAM	4382	Intergenic	LMAN1L	ENSG00000140506
chr5	179185094	179185116	+	4	TGGAAGGGATGCCCCAGCAG	AGG	||-||-||[||||--||||||]PAM	3939	Intronic	ADAMTS2	ENSG00000087116
chr8	94080361	94080383	-	4	TGAAATAGATGAGTCAGCAG	TGG	|||||--|[|||-|-||||||]PAM	17381	Intergenic	KB-1184D12.1	ENSG00000253585
chr22	49522403	49522425	+	4	TGACACCGATGGGAGAGCAG	GGG	|||-||-|[|||-||-|||||]PAM	11637	Intronic	RP4-566L20.1	ENSG00000279597
chr20	10972280	10972302	-	4	TGGAAGGGATGCCAGAGCAG	GGG	||-||-||[||||-|-|||||]PAM	14373	Intronic	RP11-103J8.1	ENSG00000270792
chr7	139023471	139023493	-	4	TGAACTGGATGCAAGAGCAG	TGG	||||--||[||||-|-|||||]PAM	2213	Intergenic	ZC3HAV1L	ENSG00000146858
chrX	18534049	18534071	-	4	TGAGACTGATGCCAGAGCAG	GGG	|||-||-|[||||-|-|||||]PAM	1008	Intronic	GJA6P	ENSG00000236187
chr20	22259176	22259198	+	4	TGAAATTGATGCAAAAGCAG	AGG	|||||--|[||||-|-|||||]PAM	3867	Intergenic	LINC01427	ENSG00000225321
chr8	98007275	98007297	+	4	TGAAACCTATGTGACTGCAG	AGG	||||||--[|||-|||-||||]PAM	48	Intronic	MATN2	ENSG00000132561
chr11	72581811	72581833	+	4	GGCAACGGATGTGACAGTAG	AGG	-|-|||||[|||-|||||-||]PAM	44	Intronic	PDE2A	ENSG00000186642
chr8	79072189	79072211	+	4	TGGAAAGGATGGGACAGGAG	AGG	||-||-||[|||-|||||-||]PAM	25152	Intergenic	AC009941.1	ENSG00000264969
chr2	9604982	9605004	+	3	TGAAATGGATGCAACAGAAG	TGG	|||||-||[||||-||||-||]PAM	10263	Intronic	Y_RNA	ENSG00000207086
chr8	28476316	28476338	+	4	AGAAACTGATGGGACAGAAG	GGG	-|||||-|[|||-|||||-||]PAM	2509	Intronic	FBXO16	ENSG00000214050
chr13	102760080	102760102	-	4	TTAAACTGATGTGACAGAAG	AGG	|-||||-|[|||-|||||-||]PAM	1010	Intergenic	CCDC168	ENSG00000175820
chrX	117570686	117570708	-	4	TAAAACTGATGGGACAGGAG	TGG	|-||||-|[|||-|||||-||]PAM	NA	Intergenic	NA	NA
chr5	53337725	53337747	+	4	TGAAAAGCATACGACAGAAG	TGG	|||||-|-[||-||||||-||]PAM	75757	Intergenic	RP11-289K10.1	ENSG00000240052
chr6	84002433	84002455	-	4	TGGAACTGATGCTACAGTAG	GGG	||-|||-|[||||-||||-||]PAM	3887	Intronic	RP4-676J13.2	ENSG00000228679
chr3	78617929	78617951	-	4	GGATACGGATGCGCCAGAAG	AGG	-||-||||[|||||-|||-||]PAM	0	Exonic	ROBO1	ENSG00000169855
chr18	74357247	74357269	+	4	AGAAATGGATGAGACAGCCG	TGG	-||||-||[|||-||||||-|]PAM	0	Exonic	C18orf63	ENSG00000206043
chr2	235025370	235025392	-	4	TGAAACCAATGCCACAGGAG	AGG	||||||--[||||-||||-||]PAM	9479	Intronic	SH3BP4	ENSG00000130147
chr10	106712134	106712156	+	4	TGAATCAGATGCCACAGCTG	AGG	||||-|-|[||||-|||||-|]PAM	2793	Intronic	SORCS1	ENSG00000108018
chr4	163913961	163913983	+	4	CGAAAGGGATGTGACAGCAC	GGG	-||||-||[|||-|||||||-]PAM	18543	Intronic	RP11-606P2.1	ENSG00000177803
chr6	13428386	13428408	+	4	TGAGAAGGATGCGAAAGCCG	AGG	|||-|-||[||||||-|||-|]PAM	20249	Intronic	GFOD1	ENSG00000145990
chr10	121570552	121570574	-	4	TGAAAAGTATGCCACAGCAA	TGG	|||||-|-[||||-||||||-]PAM	4641	Intronic	FGFR2	ENSG00000066468