There are 2 membranes in the image and only the top membrane was used.

Lane order (only lane 1 to 6 were used in the current study): 
1- Marker
2- 18075 30d = Ctrl1
3- 580 30d = Ctrl 2
4- 581 30d = Ctrl3
5- R272Q 30d = PD
6- R272Q GC 30d = iCtrl
7-17 - Not relevant for current study