Top membrane has Batch 1 & 2, lower membrane has Batch 3 & 4 TOP MEMBRANE Lane 1: Ladder Lane 2: B1 WT = Ctrl1 Lane 3: B1 R272Q = PD Lane 4: B1 R272Q-GC = iCtrl Lane 5: B2 WT = Ctrl1 Lane 6: B2 R272Q = PD Lane 7: B2 R272Q-GC = iCtrl BOTTOM MEMBRANE Lane 1: Ladder Lane 2: B3 WT = Ctrl1 Lane 3: B3 R272Q = PD Lane 4: B3 R272Q-GC = iCtrl Lane 5: B4 WT = Ctrl1 Lane 6: B4 R272Q = PD Lane 7: B4 R272Q-GC = iCtrl