Path to raw images: LCSB_HCS\YokogawaCV8000Standalone\CellPathfinder\Isabel\IR_20211129_488TH_568DCX_647MAP2_RS_e45e46e47d30_20211129_174319\11-29-21_17-49-22 To analyze them, use the image analysis scripts in LCSB_Cellular_CellularBiology\16-Our Papers\In Preparation\GBA hMO_Isabel\Scripts\Image analysis quantification The slidelayout is in the Accessory Files folder