Path to raw images: LCSB_HCS\YokogawaCV8000Standalone\CellPathfinder\Isabel\IR_20211013_488Nestin_568Sox2_647DCX_RS_e40e41e42d30_20211012_095751\10-13-21_19-11-50 To analyze them, use the image analysis scripts in LCSB_Cellular_CellularBiology\16-Our Papers\In Preparation\GBA hMO_Isabel\Scripts\Image analysis quantification The slidelayout is in the Accessory Files folder