Path to raw non-stitched images: LCSB_HCS\YokogawaCV8000Standalone\BTSData\MeasurementData\isabel\IR_20220915_488Ki67_568Edu_647Sox2_RS_e51e52e54d30_20220915_175904\09-15-22_18-04-42 To analyze them, use the image analysis scripts in LCSB_Cellular_CellularBiology\16-Our Papers\In Preparation\GBA hMO_Isabel\Scripts\Image analysis quantification The slidelayouts for each barcode in the Accessory Files folder