Path to raw images: LCSB_HCS\YokogawaCV8000Standalone\CellPathfinder\Isabel\IR_20210820_488TH_568Sox2_647MAP2_RS_e35to37e31to33d60_20210820_101636/08-20-21_10-22-02 To analyze them, use the image analysis scripts in LCSB_Cellular_CellularBiology\16-Our Papers\In Preparation\GBA hMO_Isabel\Scripts\Image analysis quantification The slidelayout is in the Accessory Files folder